![]() 6 However, because infection with ≥ 1 Ehrlichia spp induces cross-reactive antibodies and because unique WB patterns have not been consistently reported, the WB analysis cannot be performed to confirm infection by a specific Ehrlichia spp. 5 Thus, seroreactivity to E canis antigens on IFA can indicate exposure to 1 of the listed agents but not necessarily exposure to E canis.Īlthough not widely available, WB immunoassays that use native E canis antigens have been developed to confirm IFA results. 4 An IFA that uses E canis antigen as a substrate (ie, the E canis-IFA) is commonly used to screen canine sera for prior exposure to any of the 3 Ehrlichia organisms. 1-3 Sera from Ehrlichia infected dogs will typically cross-react in immunoassays. In the southeastern United States, the following 3 species of Ehrlichia are most commonly implicated: Ehrlichia canis, Ehrlichia chaffeensis, and Ehrlichia ewingii. A poor correlation was found between IFA results and those of WB analyses and the ELISA for serum samples in the low-titer group (19), with only 4 of the 19 serum samples having positive results on both WB analyses and the commercially available ELISA.Ĭonclusions and Clinical Relevance-The discrepancy between E canis-IFA, WB analyses, and the commercially available ELISA results for the low-titer serum samples may be related to a high IFA sensitivity or, more likely, a lack of specificity associated with cross-reactivity among Ehrlichia spp.Ĭanine ehrlichiosis is caused by infection with ≥ 1 tick-borne Ehrlichia spp. Results-For all serum samples in the nonreactive (n = 16), medium-titer (17), and high-titer (18) groups, correlation of results among IFA, WB analyses, and the commercially available ELISA was excellent. Correlation between results of the 3 testing modalities (ie, IFA, WB analyses, and the ELISA) was examined by use of nonreactive ( E canis-IFA reciprocal titer, 20,480) serum samples. Procedure-Serum samples were evaluated by use of 2 WB analyses and a commercially available ELISA. ![]() ![]() ![]() Sample Population-54 canine serum samples that were reactive on E canis-IFA and 16 canine serum samples that were E canis-IFA nonreactive. Objective-To examine the correlation between results for an indirect immunofluorescence assay (IFA) that uses Ehrlichia canis antigen as a substrate (ie, E canis-IFA), 2 western blot (WB) analyses, and a commercially available ELISA in the detection of E canis antibody in dog sera. ![]()
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